ABSTRACT Title of Thesis: VISUALIZATION OF CERAMIDE CHANNEL BY TRANSMISSION ELECTRON MICROSCOPY
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چکیده
Title of Thesis: VISUALIZATION OF CERAMIDE CHANNEL BY TRANSMISSION ELECTRON MICROSCOPY Soumya Samanta, Master of Science, 2011 Thesis directed by: Professor Marco Colombini Department of Biology Functional studies have shown that the sphingolipid ceramide, self-assembles in phospholipid membranes to form large channels capable of allowing proteins to cross the membrane. Here these channels are visualized by negative stain transmission electron microscopy. The images contain features consistent with stain-filled pores having a roughly circular profile. There is no indication of tilt, and the results are consistent with the formation of right cylinders. The sizes of the pores range from 5 to 40 nm in diameter with an asymmetric distribution indicating no apparent upper size limit. The size distribution matches well with the distribution of sizes calculated from electrophysiological measurements. VISUALIZATION OF CERAMIDE CHANNELS BY TRANSMISSION ELECTRON MICROSCOPY
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